EFFECT OF SOME GROWTH REGULATORS ON CALLUSING IN DALBERGIA SISSOO

Calli were obtained using hypocotyl region of scedlings of Dalbergia sisoo as the explant in MS media supplemented with auxins namely 2,4-D, NAA and IAA and cytokinin viz.: BAP used in different concentrations, singly or in combinations. Good growth of callus was obtained in NAA (0.5 mg/1)+BAP (0.5 mg/l) and BAP (0.5 mg/l)+IAA (0.5 mg/1) treatments. Cell density was found to be highest in BAP (0.5 mg/l) treatment. The greater number of cells/mg callus mass was possibly due to higher rate of cell division in cytokinin treatment.