Different Dosages of Progesterone in Luteal Phase Support Reflect Varying Endometrial microRNA Expression in Frozen Embryo Transfer Cycles

Different Dosages of Progesterone in Luteal Phase Support Reflect Varying Endometrial microRNA Expression in Frozen Embryo Transfer Cycles Wen-Jui Yang Department of Infertility and Reproductive Medicine, Taiwan IVF Group Center, Hsinchu 30274, Taiwan Department of Obstetrics and Gynaecology, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong Farn Lu Department of Infertility and Reproductive Medicine, Taiwan IVF Group Center, Hsinchu 30274, Taiwan Cai-Yun Wang Inti Labs, Hsinchu 30261, Taiwan Jun-Jie Hong Inti Labs, Hsinchu 30261, Taiwan Tiffany Wang Inti Labs, Hsinchu 30261, Taiwan Pok Eric Yang Inti Labs, Hsinchu 30261, Taiwan Jack Yu-Jen Huang Department of Infertility and Reproductive Medicine, Taiwan IVF Group Center, Hsinchu 30274, Taiwan Department of Obstetrics & Gynecology, Stanford University, Stanford, CA 94305, USA

Despite serum progesterone being a widely accepted method for luteal phase support during embryo transfer cycles, debates persist regarding the optimal strategy for guiding clinical decisions on progesterone dosages to maximize reproductive outcomes. This retrospective study explored the utility of microRNA (miRNA) biomarkers in guiding personalized progesterone dosage adjustments for frozen embryo transfer (FET) cycles in 22 in vitro fertilization (IVF) patients undergoing hormone replacement therapy. Utilizing MIRA, an miRNA-based endometrial receptivity test, we analyzed patients’ miRNA expression profiles before and after progesterone dosage adjustments to determine suitable dosages and assess endometrial status. Despite patients receiving identical progesterone dosages, variations in miRNA profiles were observed in the initial cycle, and all patients presented a displaced window of implantation. Following dosage adjustments based on their miRNA profiles, 91% of patients successfully transitioned their endometrium towards the receptive stages. However, two patients continued to exhibit persistent displaced receptivity despite the adjustments. Given the evident variation in endometrial status and serum progesterone levels among individuals, analyzing miRNA expression profiles may address the challenge of inter-personal variation in serum progesterone levels, to deliver more personalized dosage adjustments and facilitate personalized luteal phase support in IVF.
03 25 2024 3670 ijms25073670 https://creativecommons.org/licenses/by/4.0/ 10.3390/ijms25073670 https://www.mdpi.com/1422-0067/25/7/3670 https://www.mdpi.com/1422-0067/25/7/3670/pdf