Effect of Putri Malu (Mimosa pudica Linn) Leaf Extract as an Inhibitor of Vibrio cholerae Growth

Indonesia is famous for its diversity of plant sources that are beneficial for health, including the Putri Malu plant (Mimosa pudica Linn). Putri Malu leaves contain compounds such as alkaloids, flavonoids, saponins, and tannins, which are antibacterial. This research aims to determine the effect of Putri Malu leaves ethanol extract as an inhibitor against Vibrio cholerae. This research is experimental in vivo using the Kirby-Baurer disk diffusion method. The Putri Malu leaf extract concentrations used were 100 mg/ml, 50 mg/ml, 25 mg/ml, 12.5 mg/ml, 6.25 mg/ml, 3.125 mg/ml, and 1.5625 mg/ml. The antibiotic tetracycline 30 mcg was used as a positive control, and distilled water was used as a negative control. The average diameter of the Vibrio cholerae inhibition zone was 21.00 mm and 0 mm for the positive control and negative control, respectively. The average diameter of the bacterial inhibition zone for Putri Malu leaf extract was 2.36 mm, 1.26 mm, 0.96 mm, 1.30 mm, 0.80 mm, 0.60 mm, and 0.36 mm, respectively. Based on the data, it can be concluded that Putri Malu leaf extract is less effective in inhibiting Vibrio cholerae growth.