INDUCTION OF OXIDATIVE STRESS AND LYMPHOCYTE PROLIFERATION BY NANOHYDROXYAPATITE IN MICE

The aim of this study is to evaluate the potential impact of intraperitoneally administered nanohydroxyapatite on the liver oxidative status and also to study the immunogenicity of the administered nanohydroxyapatite in Swiss albino mice. Biochemical estimation of the lipid peroxidation, reduced glutathione and the activities of the Glutathione peroxidase, Glutathione reductase and the Superoxide dismutase in the liver were carried out following 7, 14 and 21 days post-treatment with nanohydroxyapatite. Tritiated thymidine uptake studies were done with the splenic lymphocytes for studying the immunogenic potential. Histopathological evaluation of the liver, spleen and the kidney was also carried out. There was marked reduction in the reduced glutathione levels and in the activities of the antioxidant enzymes GPX, GR and SOD at 7 and 14 day and further showed an increase by the 21 day. The LPO levels showed a significant increase by the 21 day. The T lymphocytes proliferated by two-fold by the 7 day and returned to normal levels by the 14 day and an increase was seen by the 21 days. There was no histopathological lesion observed in the treated groups. The nanohydroxyapatite treatment lead to a marked reduction in the levels of the antioxidant enzyme activities which however increases by the 21 day indicating that the body is able to defend against the oxidative stress mounted by the nanohydroxyapatite. Nanohydroxyapatite has immunogenic potential as it was able to elicit proliferation of the T lymphocytes up to 21 days.