THE EFFECTIVENESS OF MIXED LEAVES FROM PANDAND LEAVES (Pandanus amaryllifolius) AND LEMONGRASS STEMS (Cymbopogon nardus Linn. Rendle) AS BIOLARVACIDES AGAINST AEDES AEGYPTI LARVA

Using of chemical larvicides continuously triggers resistance in larvae and side effects for humans. Larvicides from natural ingredients are safer for health, materials derived from plants are called biolarvicides. Fragrant pandanus and lemongrass stems have the potential to be biolarvicides because they contain saponins, flavonoids, alkaloids, and polyphenols. The purpose of this study was to analyze the effectiveness of a mixture of biolarvicides from fragrant pandan leaves (Pandanus amaryllifolius) and lemongrass stems (Cymbopogon nardus Linn. Rendle) on the mortality of Aedes aegypti larvae.

This type of research is experimental with post test only control group research design, using 5 treatments. The treatments used were a mixture of fragrant pandan extract and lemongrass stems with concentrations of 10%, 15%, 20%, 25%, and 1 control. The sample used was 500 larvae of Aedes aegypti divided into 20 larvae for each group with 6 repetitions and exposure time for 24 hours and room temperature, water temperature, water pH, and humidity as control variables. Data on larval mortality was analyzed using the One Way Anova statistical test.

The mortality of Aedes aegypti larvae at concentrations of 10%, 15%, 20%, and 25%, respectively, was 28.3%, 39.1%, 50%, and 65.8% of the test larvae. The higher the concentration of the mixture of fragrant pandan leaves extract (Pandanus amaryllifolius) and lemongrass stems (Cymbopogon nardus Linn. Rendle) given, the greater the average death of Aedes aegypti larvae.

A mixture of fragrant pandan leaves extract and lemongrass stems has the potential as a biolarvicide of Aedes aegypti, but has not been effective in killing 90-100% of larvae. Further researchers can increase the concentration of the extract mixture which allows killing 90-100% of test larvae.