Assessment of Phenazone and Lidocaine Hydrochloride in Bulk and Pharmaceutical Dosage Form by Using RP-HPLC Method

An attempt has been made to develop an accurate, specific and reproducible method for the determination of Phenazone (PNZ) and Lidocaine (LID) in combined dosage forms in presence of degradation product for the content analysis during stability studies from pharmaceutical dosage form. Pharmaceutical grade of Phenazone and Lidocaine HCL kindly supplied as a gift sample by Glenmark Pharmaceutical Ltd., Nashik, India. Recovery studies by standard addition method were performed in view of justifying accuracy of the proposed methods. Previously analysed samples containing LID and PNZ was spiked with standard LID and PNZ, and the mixtures were analysed in triplicate (n=3) by proposed methods. The results of robustness testing showed that little change of method conditions, such as pH of the mobile phase, composition of the mobile phase, temperature, flow rate, and wavelength, does not affect the method significantly, and so it is robust within the acceptable limits. Percent of recovery was within the range of for LID 99.0% to 101.0% and for PNZ was found to be 99.65-101.23%. The % R.S.D. less than two values indicated robustness of the method. The proposed HPLC methodprovide simple, accurate and reproducible quantitative analysis for determination of PNZ and LID and in tablet. As the method could effectively separate the drugs from their degradation products; therefore, it can be employed as a stability indicating study.