Influences of Explant Type on in vitro Regeneration of Malaysian Chilli (Capsicum annuum L.) var CB 4

Regeneration system via organogenesis has been developed for Malaysian chilli (Capsicum annuum L) var. CB4. Hypocotyls, cotyledons and petioles from In vitro germinated seedlings were cultured on medium comprising of Murashige and Skoog’s basal medium supplemented with 5 mg/l BAP, 1 mg/l IAA and 25 g/L DJ nutrient. Hypocotyls and petioles were found to be the best explants, based on its ability to produce shoot buds compared to cotyledons. Shoot buds were elongated on MS medium containing 3 mg/l BAP, 1 mg/l IAA, 15 g/L DJ, 2 mg/L GA3, 10 mg/l AgNO3 and 15 g/L DJ nutrient. Rooting was induced on MS basal containing IAA.