GC-MS Analysis of Leaf, Fruits and Latex of Croton bonplandianum Baill

Aims: To characterize the phytocompounds of different parts of Croton bonplandianum using GC-MS.

Study Design: GC-MS analysis of bioactive constituents of C. bonplandianum.

Place and Duration of Study: Post Graduate and Research Department of Biochemistry at Government Arts College (Autonomous), Kumbakonam and Food Safety and Quality Testing Laboratory, Indian Institute of Crop Processing Technology, Thanjavur, Tamilnadu, India, between May, 2011 to June, 2012.

Methodology: The C. bonplandianum leaf and fruit (25 grams) powder was soaked in 60 ml of ethanol each and kept at room temperature for 12 hours and the fresh latex of 10 ml was mixed with 90 ml of ethanol and kept at shaker for 3 hours. The samples were filtered and concentrated. Each sample was subjected to phytochemical analysis using GC-MS.

Results: The GC-MS analysis showed peaks of twenty one phytocompounds from different parts of C. bonplandianum. Out of which five compounds were found in leaf, one compound in latex and fifteen compounds in fruits. The highest peak area of 88.69% for 16-Hexadecanoyl hydrazide and the lowest peak area of 1.39% for Phytol were obtained in leaf extract. The latex of C. bonplandianum showed that the presence of Myo-Inositol, 2-C-methyl with the peak area of 30.8%. The fruits of C. bonplandianum showed that the presence of 9, 12, 15-Octadecatrienoic acid, methyl ester (z,z,z)- with the highest peak area of 41.81% and 2-Hexen-1-ol, 2-ethyl with the lowest peak area of 0.69%.

Conclusion: The phytochemical constituents of C. bonplandianum have been screened and the isolation of individual bioactive compounds from C. bonplandianum will be helpful to find new drugs.