Colnaghi, Luca and Russo, Luca and Natale, Carmina and Restelli, Elena and Cagnotto, Alfredo and Salmona, Mario and Chiesa, Roberto and Fioriti, Luana (2019) Super Resolution Microscopy of SUMO Proteins in Neurons. Frontiers in Cellular Neuroscience, 13. ISSN 1662-5102
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Abstract
The ubiquitously expressed SUMO proteins regulate a plethora of cellular pathways and processes. While they have a predominantly nuclear localization, extranuclear roles of SUMO isoforms at the synapse have also been described, making SUMOylation one of the major post-translational regulators of nerve functions. These findings have however recently been challenged, at least for SUMO1, by the analysis of knock-in mice expressing His6-HA-SUMO1, where the authors failed to detect the protein at the synapse. In the ongoing dispute, the subcellular distribution in neurons of SUMO2/3 and of the E2 SUMO ligase Ubc9 has not been examined. To investigate whether SUMO proteins do or do not localize at the synapse, we studied their localization in hippocampal primary neurons by super resolution microscopy. We found that SUMO1, SUMO2/3, and Ubc9 are primarily nuclear proteins, which also colocalize partially with pre- and post-synaptic markers such as synaptophysin and PSD95.
Item Type: | Article |
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Subjects: | Euro Archives > Medical Science |
Depositing User: | Managing Editor |
Date Deposited: | 25 May 2023 11:15 |
Last Modified: | 01 Dec 2023 03:59 |
URI: | http://publish7promo.com/id/eprint/2605 |