Xia, Lei and Xu, Jianhua and Song, Juan and Xu, Yiran and Zhang, Bohao and Gao, Chao and Zhu, Dengna and Zhou, Chongchen and Bi, Dan and Wang, Yangong and Zhang, Xiaoli and Shang, Qing and Qiao, Yimeng and Wang, Xiaoyang and Xing, Qinghe and Zhu, Changlian (2019) Autophagy-Related Gene 7 Polymorphisms and Cerebral Palsy in Chinese Infants. Frontiers in Cellular Neuroscience, 13. ISSN 1662-5102
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Abstract
Cerebral palsy (CP) is a group of non-progressive motor impairment syndromes that are secondary to brain injury in the early stages of brain development. Numerous etiologies and risk factors of CP have been reported, and genetic contributions have recently been identified. Autophagy has an important role in brain development and pathological process, and autophagy-related gene 7 (ATG7) is essential for autophagosome biogenesis. The purpose of this study was to investigate the genetic association between ATG7 gene single nucleotide polymorphisms (SNPs) and CP in Han Chinese children. Six SNPs (rs346078, rs1470612, rs11706903, rs2606750, rs2594972, and rs4684787) were genotyped in 715 CP patients and 658 healthy controls using the MassArray platform. Plasma ATG7 protein was determined in 73 CP patients and 79 healthy controls. The differences in the allele and genotype frequencies of the rs1470612 and rs2594972 SNPs were determined between the CP patients and controls (pallele = 0.02 and 0.0004, pgenotype = 0.044 and 0.0012, respectively). Subgroup analysis revealed a more significant association of rs1470612 (pallele = 0.004, pgenotype = 0.0036) and rs2594972 (pallele = 0.0004, pgenotype < 0.0001) with male CP, and more significant differences in allele and genotype frequencies were also noticed between CP patients with spastic diplegia and controls for rs1470612 (pallele = 0.0024, pgenotype = 0.008) and rs2594972 (pallele < 0.0001, pgenotype = 0.006). The plasma ATG7 level was higher in CP patients compared to the controls (10.58 ± 0.85 vs. 8.18 ± 0.64 pg/mL, p = 0.024). The luciferase reporter gene assay showed that the T allele of rs2594972 SNP could significantly increase transcriptional activity of the ATG7 promoter compared to the C allele (p = 0.009). These findings suggest that an association exists between genetic variants of ATG7 and susceptibility to CP, which provides novel evidence for the role of ATG7 in CP and contributes to our understanding of the molecular mechanisms of this neurodevelopmental disorder.
Item Type: | Article |
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Subjects: | Euro Archives > Medical Science |
Depositing User: | Managing Editor |
Date Deposited: | 26 May 2023 04:25 |
Last Modified: | 16 Sep 2023 04:01 |
URI: | http://publish7promo.com/id/eprint/2604 |