Endosomal Wnt signaling proteins control microtubule nucleation in dendrites

Weiner, Alexis T. and Seebold, Dylan Y. and Torres-Gutierrez, Pedro and Folker, Christin and Swope, Rachel D. and Kothe, Gregory O. and Stoltz, Jessica G. and Zalenski, Madeleine K. and Kozlowski, Christopher and Barbera, Dylan J. and Patel, Mit A. and Thyagarajan, Pankajam and Shorey, Matthew and Nye, Derek M. R. and Keegan, Matthew and Behari, Kana and Song, Song and Axelrod, Jeffrey D. and Rolls, Melissa M. and Akhmanova, Anna (2020) Endosomal Wnt signaling proteins control microtubule nucleation in dendrites. PLOS Biology, 18 (3). e3000647. ISSN 1545-7885

[thumbnail of file_id=10.1371%2Fjournal.pbio.3000647&type=printable] Text
file_id=10.1371%2Fjournal.pbio.3000647&type=printable - Published Version

Download (6MB)

Abstract

Dendrite microtubules are polarized with minus-end-out orientation in Drosophila neurons. Nucleation sites concentrate at dendrite branch points, but how they localize is not known. Using Drosophila, we found that canonical Wnt signaling proteins regulate localization of the core nucleation protein γTubulin (γTub). Reduction of frizzleds (fz), arrow (low-density lipoprotein receptor-related protein [LRP] 5/6), dishevelled (dsh), casein kinase Iγ, G proteins, and Axin reduced γTub-green fluorescent protein (GFP) at branch points, and two functional readouts of dendritic nucleation confirmed a role for Wnt signaling proteins. Both dsh and Axin localized to branch points, with dsh upstream of Axin. Moreover, tethering Axin to mitochondria was sufficient to recruit ectopic γTub-GFP and increase microtubule dynamics in dendrites. At dendrite branch points, Axin and dsh colocalized with early endosomal marker Rab5, and new microtubule growth initiated at puncta marked with fz, dsh, Axin, and Rab5. We propose that in dendrites, canonical Wnt signaling proteins are housed on early endosomes and recruit nucleation sites to branch points.

Item Type: Article
Subjects: Euro Archives > Biological Science
Depositing User: Managing Editor
Date Deposited: 30 Jan 2023 04:20
Last Modified: 05 Apr 2024 05:41
URI: http://publish7promo.com/id/eprint/1417

Actions (login required)

View Item
View Item