Diatta, Charlot and Diassy, Henry and Barboza, Firmin Sylva and Ball, Fatimata Seydy and Camara, Mariama and Gassama, Abdoulaye and Yoro, S. Y. Guata (2022) Effects on Blood Glucose of Ethyl Acetate-Butanol Fraction of Dialium guineense Aqueous Leaf Extract (Cesalpiniaceae). Journal of Pharmaceutical Research International, 34 (56). pp. 77-86. ISSN 2456-9119
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Abstract
Introduction: The overall objective of the study was to evaluate the effect on blood glucose of the ethyl acetate-butanol fraction (EABF) compounds of the aqueous leaf extract of Dialium guineense (Cesalpiniaceae).
Materials and Methods: The powder of D. guineense leaves was subjected to decoction. The aqueous solution was respectively fractionated with ethyl acetate and butanol. The sub-fractions were grouped to form the ethyl acetate-butanol fraction (EABF), enriched in flavonoids and tannins. The condensed tannins of EABF were eliminated under the action of casein, resulting in a condensed tannin-free fraction (EAB-TFF). EABF and EAB-TFF were phytochemically characterized and tested in normo-glycemic rats, a glucose tolerance test and type 2 diabetic rats.
Results, Analysis and Discussion: EABF (300 mg/kg, per os) has no significant effect on blood glucose in normo-glycemic rats (0.80±0.12 vs 0.81±0.01 g/L). Under the same conditions, EABF without tannins (EAB-TFF: 300 mg/kg, per os) is hypoglycemic (0.57±0.05 vs 0.82±0.05 g/L). These results suggest the existence of an antagonism, in the effects on blood glucose, between tannins and probably flavonoid-like compounds. In type 2 diabetic rats, the daily administration of EABF (300 mg/kg/day, per os) varied blood glucose from 2.73±0.39 to 1.14±0.58 g/L (n=5, p<0.05). Similar effects were observed with EAB-TFF (300 mg/kg/day, per os), administered under the same conditions (1.12±0.04 g/L vs 3.01±0.5 g/L) (p<0.05, n=5). EABF has no effect on blood glucose in normo-glycemic rats, whereas under the same conditions, EAB-TFF induces an hypoglycemic effect. These results suggest the existence of compounds acting in opposite directions, in the regulation of blood glucose.
Item Type: | Article |
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Subjects: | Euro Archives > Medical Science |
Depositing User: | Managing Editor |
Date Deposited: | 19 Dec 2022 06:34 |
Last Modified: | 01 Jan 2024 12:26 |
URI: | http://publish7promo.com/id/eprint/1020 |